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To evaluate the virulence genes and antibiotics resistance of Staphylococci species in mastitis cows isolated from parts of Guangdong,and then provide scientific basis of the prevention of bovine mastitis.Forty strains Staphylococci isolated from milk samples of 110 mastitis cows were collected,and virulence genes,resistance genes and disinfectant resistant genes were detected by PCR,and antibiotics resistance with K-B paper method.The results showed that the highest virulence gene was fnbp (17.5％),followed by seb (15％) and tsst (15％),and virulence genotype was complex.The most prevalent antibiotic resistance drug wvas streptomycin,followed by erythromycin and penicillin G,and CNS were susceptible to oxacillin,ceftraxone and cefazolin.The most prevalent antibiotic resistance genes was quinolones gene qnrA/B/C/D(20％),the resistance to streptomycin was mediated by aac6-aph2.The most prevalent disinfectant resistant gene was qacG (225 ％).The genotype of antibiotics resistance gene and disinfectant resistant gene was complex.
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Objective:To observe the characteristics of the tissue distribution of the blood brain barrier through the modification of the liposome encapsulated brain derived neurotrophic factor.Methods: A total of 32 SD rats were selected from Xi′an Medicine College of Foreign Affairs as the research object,and randomly divided into two groups (n=16) and control group (n=16).To establish a rat model of AD rats in the control group received no treatment,liposome group by rat tail vein injection of 10 μg/kg Tf-pGFAP-BDNF-PEG,analysis of liposome entrapped BDNF targeting liposomes through blood brain barrier tissue distribution characteristics.Results: 40-50 nerve growth factor (1 ml) was collected by using 24mCi99mTC and 20 L 0.5 ml was collected on the top of the column.The concentration of 8-17 tube in the peak was recovered,and the results showed that the labeling rate of neurotrophic factor was 98.9％.Liposome group for tenth days,14 days of BDNF immunoreactive cells,significantly higher than the control group (P<0.05);neurotrophic factor in brain tissue of rats in body lipid gene expression at day fourteenth,significantly higher than the control group (P<0.05);expression of neurotrophic factors in liposome group rat,and neurotrophic factor expression level was significantly higher than the control group (P<0.05).The cerebrospinal fluid was clear,colorless and transparent,and no red blood cells were detected under microscope.Liposome group in cerebrospinal fluid of rats with fecal occult blood detection results of gold colloid showed negative,while the control group was positive.Conclusion: The combination of transferrin and polyethylene glycol modified liposomes combined with brain specific promoter can improve the targeting of liposomes.
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Purpose To investigate the relevance between the expression of miR-339-5p and the clinicopathological characteristics in diffuse large B-cell lymphoma (DLBCL). Methods The level of miR-339-5p expression was detected in 123 cases of diffuse large B-cell lymphoma tissues and 20 cases of reactive lymphoid hyperplasia tissues by chromogenic in situ hybridization ( CISH) technique. The expression of Ki-67 and BCL-6 protein was examined in diffuse large B-cell lymphoma tissues by immunohistochemical technique (IHC) (EnVision two-steps), and the correlation between the expression of miR-339-5p and BCL-6 and the clinicopathological param-eters was also analyzed. Results The positive rates of miR-339-5p were 39. 8% (49/123) in DLBCL tissues, which was significantly lower than that in RH tissues (90%, 18/20). The positive rates of miR-339-5p were 31. 0% (22/71) for germinal center B-cell-like (ABC type) DLBCL, which was significantly lower than that in activated B-cell-like (GCB type) DLBCL (27/52, 51. 9%). The low-er expression of miR-339-5p in DLBCL was correlated with late Ann Arbor staging and high-risk group of international prognostic index (P<0. 05). The survival rates of miR-339-5p negative patients of ABC type and GCB type of DLBCL were significantly lower than that of the positive patients (P<0. 01). The levels of miR-339-5p expression in DLBCL were negatively correlated with the levels of BCL-6 expression in DLBCL (P<0. 01). Conclusion The low expression of miR-339-5p might be relatived with the progression and poor prognosis of DLBCL.
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Improvement in lung function was reported after acupuncture treatment of chronic obstructive pulmonary disease (COPD), but little is known about the underlying mechanisms. Because an immune response imbalance could be seen in COPD, we hypothesize that electroacupuncture (EA) may play a role in regulating inflammatory cytokines and contribute to lung protection in a rat model of smoke-induced COPD.
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After sequencing, we amplified and cloned foot-and-mouth disease virus (FMDV) O/QYYS/s/06 whole genome by three fragments. These three fragments were cloned into vector P43 one by one to construct recombinant plasmid P43C, which carried the full-length cDNA of FMDV O/QYYS/s/06. Then, plasmid P43C and plasmid T7 expressing T7 RNA polymerase were co-transfected into BHK-21 cells. After 48 h, we harvested the culture broth from transfected BHK-21 cells and inoculated into 2-3 day-old sucking mice. After four generation passage, the virus harvested from sucking mice was confirmed to be type O FMDV by the indirect hemagglutination test, sucking mice's neutralization test and sequencing. The results showed that we have successfully constructed the full-length cDNA clone of FMDV O/QYYS/s/06 strain.
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Animals , Mice , Animals, Newborn , Cloning, Molecular , DNA, Complementary , Genetics , DNA, Viral , Genetics , Foot-and-Mouth Disease , Virology , Foot-and-Mouth Disease Virus , Classification , Genetics , Virulence , Transcription, Genetic , TransfectionABSTRACT
objective To observe whether the killing effect on HCC SMMC-7721 cell of the antihepatocellular carcinoma scFv reconstructed by pharmacy was enhanced or not.Methods Prokarycytic expression vector containing PET32a-RC-RNase was induced to express by IPTG.The inclusion body purified and Western-blotting was used.PC.CHOL and CHS was added in chloroform.Dry membrane was formed after chloroform was removed.RC-RNase protein solution was added to dissolute the membrane.Then pass the solution over a Sephadex G-50 column after ultrasound and filtrated to detect the encapsulation efficiency of the liposome.The solution reacted in EDC.SSNHS and MES for 30 minutes.Then add hdscFv to the solution in 4 ℃ over night.MTT method was used to detect the killing effect on HCC cell of immunoliposome RC-RNase,immunotoxin RC-RNase and liposome RC-RNase in vitro.Resuits The killing effect on HCC cell of immunoliposome RC-RNase is the best.but that of Iiposome RC-RNase is the worst.The respective JC50 are:3.28μg/ml,22.44μg/ml and 98.26μg/ml.Conclusion The anti-hepatocellular carcinoma scFv relomtructed by pharmacy can promote the killing effect on HCC cell and may have potential in the treatment of hepatocarcinoma.
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Objective:To compare the killing effects on HCC SMMC-7721 cells of immunoliposome PE38、immunotoxin PE38 and liposome PE38. Methods:Dissolute PC,CHOL and CHS in chloroform in proportion.Dry membrane was formed after chloroform was removed.Add the protein solution of PE38 to dissolute the dry membrane.Then pass the solution over a Sephadex G-50 column after ultrasoned and filtrated to detect the encapsulation efficiency of the liposome.The solution reacted in EDC,SSNHS and MES for 30 minutes.Then add Ab to the solution in 4℃ over night.MTT method was used to detect the killing effects on HCC cells of immunoliposome PE38、immunotoxin PE38 and liposome PE38 in vitro. Results:The killing effects on HCC cells of immunoliposome PE38 is the best,and that of liposome PE38 is the worst.The respective IC_(50) s are:0.59 ?g/ml、6.04 ?g/ml and 92.07 ?g/ml. Conclusion:Immunoliposome PE38 may be a protential durg in the treatment of hepatocarinoma.
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AIM To investigate whether melatonin improve the learning and memory dysfunction in the amnesic rats induced by amyloid ? peptide 25～35 (A? 25～35 ) via cholinergic nervous system or not.Methods The amnesic model in adult rats was induced by injection of A? 25～35 into hippocampus; Morris water maze was used to determine the effects of A? 25～35 and melatonin on the learning and memory. The activity of the choline acetyltransferase and acetylcholinesterase were determined by immunohistochemistry and spectrophotometry respectively. RESULTS Injection of A? 25～35 20 ?ginto the adult rats hippocampus induced learning and memory dysfunction, and a decrease in the number of ChAT immunoreactive neurons in hippocampus. Melatonin (0 1, 1, and 10 mg?kg -1 , ig?10 d) improved the A? 25～35 treated rats cognitive function and increased the number of ChAT immunoreactive neurons in hippocampus. CONCLUSION Improvement of the cholinergic dysfunction by melatonin in adult rats induced by amyloid ? peptide 25～35 may be via cholinergic nervous system.